Monkeypox Virus (MPXV) IgG ELISA Kit

Product Description

This ELISA kit uses Indirect-ELISA as the method to qualitatively detect theMonkeypox Virus (MPXV) IgG in the sample. The micro ELISA plate provided inthis kit is pre- coated with purified MPXV antigen, after adding samples to wells, theMPXV IgG in the samples will combine with the pre-coated MPXV antigen. Afterwashing completely, add Horseradish Peroxidase (HRP) conjugated goat anti humanIgG to develop the antigen- antibody-HRP conjugated secondary antibody complex.Free components are washed away, then the substrate solution is added to each well.Only those wells that contain MPXV IgG and HRP conjugated anti-human IgG willappear blue in color. The enzyme- substrate reaction is terminated by the addition ofstop solution and the color turns yellow.The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. Compared with the CUT OFF value to judge whether MPXV IgG exists inthe tested samples or not.